Isolation and identification of a new set of microsatellite loci from Ucides cordatus genome.
Isolation and identification of a new set of microsatellite loci from Ucides cordatus genome.
Author(s): ARAUJO, E. S.; BRITTO, F. B.; CARVALHO, A. M. F.; ORASMO, G. R.; DINIZ, F. M.
Summary: A new set of microsatellite loci (simple sequence repeats, SSRs) from the overexploited mangrove crab Ucides cordatus is described in this study. Microsatellite isolation used a highly simplified and inexpensive protocol based on (i) multiple enzyme digestion/ligation; (ii) mixed biotin-labeled probes and streptavidin-coated magnetic bead hybridization capture strategy, and (iii) a double-repeat-enrichment procedure...
Publication year: 2015
Types of publication: Journal article
Unit: Embrapa Mid-North
Keywords: Diversidade genética, Marcador Molecular
Observation
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