Improving protein extraction from Brachiaria ruziziensis leaf samples by using a plant protease inhibitor cocktail and a chaotropic reagent.
Improving protein extraction from Brachiaria ruziziensis leaf samples by using a plant protease inhibitor cocktail and a chaotropic reagent.
Author(s): PASSOS, L. P.; SUZUKI, L. S.; MARQUES, R.; PINTO, I. S. B.; SANTISTEVAN, G.; COUTINHO, P. R. C.; FONSECA, I.; MARTINS, M. F.
Summary: The purpose of this study was to compare a traditional, buffer-based protein extraction protocol with the utilization of a protease-blocking protein extraction kit, aiming at allowing protein manipulation under room temperature prior to proteomic approaches.
Publication year: 2011
Types of publication: Abstract in annals or event proceedings
Unit: Embrapa Dairy Cattle
Keywords: Brachiaria Ruziziensis
Observation
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